Salamanders

Heidelberg 2023, kt., XIV 483 Seiten, 89 Farbfotos, 14 sw.-Abb. This volume provides a com-prehensive reference for researchers aiming to bring new techniques and approaches to their scientific research using urodeles. Chapters are authored by leaders in the field and meant to guide readers through laboratory colony husbandry, traditional molecular techniques, experi-mental manipulation and surgeries, bioinformatics and genomics, transgenics and lineage-tracing, and physiological and organismal techniques. In addition to laboratory methods, this volume highlights techniques developed for field studies and work with wild-caught animals. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Mit den Teilen Salamanders as Key Models for Development and Regeneration Research, Establishing a New Research Axolotl Colony, Health Monitoring for Laboratory Salamanders, Husbandry, Captive Breeding, and Field Survey of Chinese Giant Salamander (Andrias davidianus), Whole-Mount In Situ Hybridization (WISH) for Salamander Embryos and Larvae, Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue, Ethyl Cinnamate-Based Tissue Clearing Strategies, Induction and Characterization of Cellular Senescence in Salamanders, Methods for Studying Appendicular Skeletal Biology in Axolotls, Fluorescence In Situ Hybridization of DNA Probes on Mitotic Chromosomes of the Mexican Axolotl, The Use of Small Molecules to Dissect Developmental and Regenerative Processes in Axolotls, In Vivo and Ex Vivo View of Newt Lens Regeneration, Bead Implantation and Delivery of Exogenous Growth Factors, The Accessory Limb Model Regenerative Assay and Its Derivatives, Embryonic Tissue and Blastema Transplantations.